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目的探讨三氧化二砷(ATO)对BXSB狼疮小鼠生存时间、外周血抗dsDNA抗体水平及离体脾脏单个核细胞白细胞介素(IL)-4、干扰素(IFN)-γ分泌水平的影响。方法BXSB狼疮小鼠44只,随机分为2组,治疗组予腹腔内注射ATO,对照组注射等量生理盐水,酶联免疫吸附试验(ELISA)测其外周血抗dsDNA抗体水平,并将生存时间作比较:另外,通过体外培养脾脏单个核细胞比较C57和BXSB小鼠脾脏单个核细胞分泌IL-4、IFN-γ水平的差异,然后观察ATO对BXSB小鼠脾脏单个核细胞IL-4、IFN-γ分泌水平的影响,同时观察ATO对细胞活性的影响。结果ATO可延长BXSB狼疮小鼠的生仔时间,显著降低其外周血中抗dsDNA抗体的水平:1μmol/L浓度的ATO对小鼠脾脏单个核细胞的活性无明显影响:与C57小鼠相比,BXSB很疮小鼠脾脏单个核细胞在体外经有丝分裂原刺激之后分泌IL-4、IFN-γ水平较高,ATO能显著抑制其分泌。结论ATO可延长BXSB狼疮鼠的生仔时间,降低其外周血抗dsDNA抗体的水平,提示ATO对BXSB狼疮鼠有一定的治疗作用:ATO抑制该小鼠脾脏单个核细胞IL-4和IFN-γ的分泌可能是其发挥治疗作用的重要机制之一。
Objective To investigate the effect of arsenic trioxide (arsenic trioxide) on the survival of BXSB lupus mice, the level of anti-dsDNA antibody in peripheral blood and the levels of interleukin (IL) -4 and IFN-γ in splenic mononuclear cells in vitro. Methods Forty-four BXSB lupus mice were randomly divided into two groups. The treatment group was given intraperitoneal injection of ATO, the control group was injected with normal saline, the level of anti-dsDNA antibody in peripheral blood was measured by enzyme linked immunosorbent assay (ELISA) Time were compared: In addition, spleen mononuclear cells were cultured in vitro compared C57 and BXSB spleen mononuclear cells secreted IL-4, IFN-γ levels were different, and then observed ATO on BXSB mouse spleen mononuclear cells IL-4, IFN-γ secretion levels, while observing the ATO on cell activity. Results ATO prolonged the life span of BXSB lupus mice and significantly decreased the level of anti-dsDNA antibody in peripheral blood. ATO at a concentration of 1 μmol / L had no significant effect on the activity of splenic mononuclear cells in mice: compared with C57 mice , BXSB is very sore mouse spleen mononuclear cells secreted IL-4 after stimulated by mitogens in vitro, the level of IFN-γ is higher, ATO can significantly inhibit its secretion. CONCLUSION ATO prolongs the life span of BXSB lupus mice and decreases the level of anti-dsDNA antibody in peripheral blood. It suggests that ATO may have some therapeutic effects on BXSB lupus mice: ATO inhibits IL-4 and IFN-γ The secretion may be one of the important mechanisms of its therapeutic effect.