论文部分内容阅读
目的研究不同类型冠心病患者外周循环中树突状细胞(dendritic cells,DCs)数量和功能的变化,探讨DCs与冠脉粥样硬化斑块从稳定到易损的关系。方法将62例冠心病患者分为稳定型心绞痛组(SAP组,17例)、不稳定型心绞痛组(UAP组,15例)、急性冠脉综合征组(ACS组,15例)、急性心肌梗死组(AMI组,15例),冠状动脉造影阴性患者为对照组(CTL组,18例)。流式细胞4色分析法检测DCs及亚型的比例,流式细胞仪及酶联免疫吸附实验(ELISA)检查DCs功能。结果(1)与CTL组比较,SAP组外周血DCs血白细胞的比例和数量无显著变化;UAP组DCs比例和数量显著增高;AMI组DCs比例和数量均减少;ACS组DCs比例降低,数量无显著改变。(2)冠心病患者外周血单核细胞源性DCs功能测定:①培养第7天,UAP、ACS、AMI组DCs CD86表达高于CTL、SAP组,ACS、AMI组显著高于UAP组(P<0.01)。②培养第7天,在DCs数量为2×105/mL时,UAP、ACS、AMI组DCs刺激T细胞增殖的能力显著高于CTL、SAP组(P<0.01),ACS、AMI组显著高于UAP组(P<0.01),AMI组较ACS组有升高趋势。③混合淋巴细胞反应上清液中IL-12、IFN-α浓度比较,UAP、ACS、AMI组显著高于CTL、SAP组;ACS、AMI组显著高于UAP组(P<0.01);AMI组高于ACS组(P<0.05)。结论冠心病患者外周血DCs数量、比例及功能可一定程度反映冠心病患者冠脉斑块稳定性的变化。
Objective To study the changes of the number and function of dendritic cells (DCs) in peripheral blood of patients with different types of coronary heart disease and to explore the relationship between DCs and coronary atherosclerotic plaque from stable to vulnerable. Methods Sixty-two patients with coronary heart disease were divided into stable angina pectoris group (SAP group, 17 cases), unstable angina pectoris group (UAP group, 15 cases), acute coronary syndrome group (ACS group, 15 cases) The infarction group (AMI group, 15 cases) and the negative coronary angiography group as the control group (CTL group, 18 cases). Flow cytometry was used to detect the ratio of DCs and subtypes. Flow cytometry and enzyme-linked immunosorbent assay (ELISA) were used to examine the function of DCs. Results (1) Compared with CTL group, there was no significant change in the proportion and number of white blood cells in peripheral blood of SAP group; the proportion and number of DCs in UAP group were significantly increased; the proportion and number of DCs in AMI group were decreased; Significantly changed. (2) Monocyte-derived DCs function in peripheral blood of patients with coronary heart disease: ①The expression of CD86 on DCs in UAP, ACS and AMI group was higher than that in CTL group on the 7th day after culture <0.01). ② On the 7th day after culture, DCs stimulated the proliferation of T cells in DCs of UAP, ACS and AMI at a dose of 2 × 105 / mL for DCs (P <0.01), significantly higher than those in ACS and AMI groups UAP group (P <0.01), AMI group than ACS group increased. (3) The levels of IL-12 and IFN-α in mixed lymphocyte reaction supernatant were significantly higher in UAP, ACS and AMI groups than those in CTL and SAP groups; the levels in ACS and AMI groups were significantly higher than those in UAP groups (P <0.01) Higher than ACS group (P <0.05). Conclusion The number, proportion and function of DCs in peripheral blood of patients with coronary heart disease may reflect the changes of coronary plaque stability to some extent.