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目的顺铂是膀胱癌化疗的一线药物,但部分患者在使用后可发生心脏毒性。该作用多由顺铂引起的细胞凋亡、氧化应激、内质网应激等引起。山奈酚是一种常见的黄酮类化合物,该药具有抗氧化、抗心脏缺血-再灌注损伤的作用。本研究主要探讨山奈酚对顺铂引起心脏毒性的保护作用。方法与结果采用顺铂联合山奈酚处理H9c2心肌细胞系及原代乳大鼠心肌细胞,发现山奈酚可显著抑制顺铂引起的细胞活力下降。TUNEL结果显示,顺铂联合山奈酚处理组TUNEL阳性细胞比例较单纯顺铂处理组显著降低。山奈酚还可显著抑制顺铂诱导的心肌细胞Caspase3剪切体表达水平。然而,在膀胱癌T24细胞中,山奈酚可显著增强顺铂对该细胞的杀伤作用。结论山奈酚对顺铂诱导的心肌细胞凋亡有抑制作用。因此,山奈酚很可能是一种新的膀胱癌顺铂治疗辅助药物。
Cisplatin is a first-line drug for bladder cancer chemotherapy, but some patients may experience cardiotoxicity after use. This effect is mostly caused by cisplatin-induced apoptosis, oxidative stress, endoplasmic reticulum stress and so on. Kaempferol is a common flavonoid compound that has antioxidant and anti-ischemic-reperfusion injury effects. This study mainly discusses the protective effects of kaempferol on cardiotoxicity induced by cisplatin. Methods and Results Cisplatin combined with kaempferol treatment of H9c2 cardiomyocytes and primary rat myocardial cells, found that kaempferol can significantly inhibit cisplatin-induced cell viability decreased. The results of TUNEL showed that the proportion of TUNEL positive cells in cisplatin combined with kaempferol treatment group was significantly lower than that in cisplatin alone treatment group. Kaempferol also significantly inhibited cisplatin-induced expression of Caspase3 splicing in cardiomyocytes. However, kaempferol in bladder cancer T24 cells significantly enhanced the killing effect of cisplatin on this cell. Conclusion Kaempferol has an inhibitory effect on cisplatin-induced cardiomyocyte apoptosis. Therefore, kaempferol is likely to be a new adjunct to cisplatin in bladder cancer.