为发掘柑橘果实脱落过程中的关键基因,构建了受乙烯诱导的柑橘果实离层SSH文库,经蓝、白斑筛选,菌液PCR检测和测序验证,共获得385条有效EST.经聚类拼接得到单基因簇30个,其中片段重叠群28个、单一EST序列2个.经BLASTX比对NCBI的非冗余蛋白数据库,在这些ESTs中,28个找到同源序列,2个无匹配;BLAST2GO功能注释表明:这些单一基因主要涉及衰老、抗逆防御、细胞壁水解、信号转导等功能. In order to find out the key genes in the process of citrus fruit exfoliation, an SSH library of ethylene-induced citrus fruit was constructed, and 385 effective ESTs were obtained by blue and white spot screening, bacterial PCR and sequencing. There were 30 single gene clusters, including 28 contigs and 2 single EST sequences.Compared with NCBI non-redundant protein database by BLASTX, 28 out of these ESTs found homologous sequences and 2 did not match; BLAST2GO function The comments indicate that these single genes are primarily involved in functions such as aging, resistance to defenses, cell wall hydrolysis, and signal transduction.