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目的：建立血浆中华法林对映体浓度的立体选择性高效液相色谱法。方法：血浆标本经碱洗、酸化和乙醚提后，与碳苄氧基－Ｌ－脯氨酸进行柱前衍生化为差向体。以正己烷－乙酸乙酯－乙酸－甲醇为流动相，经普通硅胶柱分离对映体和在紫外λ３１３ｎｍ处检测。结果：对映体标准曲线范围０～４．０ｍｇ／Ｌ，最低检出限＜０．１ｍｇ／Ｌ，批内，批间平均ＲＳＤ均＜７％，平均回收率１００．２２％，临床上常用药物对本法没有干扰。结论：本法简便可靠，可用于临床华法林对映体的药动学和药效学研究。
Objective: To establish a stereoselective HPLC method for determination of warfarin enantiomers in plasma. Methods: The plasma samples were subjected to precolumn derivatization with epi-benzaldehyde, L-proline and acidified with diethyl ether, and then differentiated into epimer. The enantiomers were separated on a normal silica gel column with n-hexane-ethyl acetate-acetic acid-methanol as mobile phase and detected at λ313 nm. Results: The standard curve of enantiomers ranged from 0 to 4.0 mg / L with the minimum detectable limit of <0.1 mg / L. The mean RSD between batches and batches was <7% and the average recovery was 100.22% Medicines do not interfere with this law. Conclusion: This method is simple and reliable, and can be used to study the pharmacokinetics and pharmacodynamics of warfarin enantiomers.