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目的观察硒对氧化偶氮甲烷(AOM)所致结肠癌大鼠睾丸精原细胞P16蛋白表达的影响。方法随机将20只3周龄断乳雄性SD大鼠分为:正常对照组、实验对照组、AOM致癌前喂食亚硒酸钠水组、AOM致癌后喂食亚硒酸钠水组。每周腹腔注射AOM 15 mg/kg,持续2周,造成大鼠结肠癌模型。用4 mg/L Na2SeO3水溶液分别在AOM致癌前、后开始进行干预,并持续至实验结果。各组均于34周后取两侧睾丸,用免疫组织化学方法,观察大鼠睾丸精原细胞内P16蛋白的表达,并进行图像分析。结果正常组大鼠的睾丸内未见精原细胞表达P16蛋白,而实验对照组、硒干预AOM致癌组(AOM致癌前、后喂食亚硒酸钠水)的精原细胞P16均呈阳性表达,阳性产物呈深棕色,定位于细胞核中,细胞质内仅有少量表达。实验对照组、AOM致癌前、后喂食亚硒酸钠水组阳性表达逐渐增强,组间具有显著性差异(P<0.05)。结论硒可增强AOM所致结肠癌大鼠精原细胞P16蛋白的表达。
Objective To observe the effect of selenium on the expression of P16 protein in testicular germ cells of rats with colon cancer induced by oxymethane methane (AOM). Methods Twenty male SD rats were randomly divided into normal control group, experimental control group, AOM pre-carcinogenic sodium selenite water group, AOM carcinogenicity after feeding sodium selenite water group. Intraperitoneal injection of AOM 15 mg / kg per week for 2 weeks resulted in a rat model of colon cancer. Interventions were started with 4 mg / L Na2SeO3 aqueous solution before and after AOM carcinogenesis, respectively, and continued to the experimental results. The testis in both groups was taken after 34 weeks. The expression of P16 protein in testicular spermatogonia was observed by immunohistochemical method and the image analysis was performed. Results No P16 protein was expressed in spermatogonia in the testis of rats in normal group, P16 in spermatogonia of experimental control group and selenium intervention AOM carcinogenic group (AOM pre-cancer and post-feeding sodium selenite water) were positive, The positive product was dark brown, located in the nucleus, only a small amount of cytoplasmic expression. In the experimental control group, the positive expression of AMS in the pre-and post-carcinogenic sodium selenite water group increased gradually, with significant difference between the two groups (P <0.05). Conclusion Selenium can enhance the expression of P16 protein in spermatogonia of AOM-induced colon cancer rats.