论文部分内容阅读
目的:建立以高效液相色谱法同时测定降脂灵片中橙黄决明素、芦荟大黄素、大黄酸、黄决明素、决明素、大黄素、大黄酚、大黄素甲醚的含量测定方法。方法:采用Phenomsil-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%磷酸水溶液,梯度洗脱,流速1.0 mL·min-1,检测波长284 nm,柱温30℃。结果:在本色谱条件下,橙黄决明素、芦荟大黄素、大黄酸、黄决明素、决明素、大黄素、大黄酚、大黄素甲醚的进样量分别在0.10~0.50,0.066~0.33,0.037~0.19,0.019~0.093,0.028~0.14,0.24~1.2,0.11~0.55、0.17~0.87μg与色谱峰面积呈良好的线性关系;加样回收率(n=6)均在97.0%~102.9%,RSD均<2.0%。结论:该法快速、准确,重复性好,可以满足降脂灵片的含量测定要求。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of orange and yellow cassia seed, aloe emodin, rhein, Huang Ding Ming Su, Cassia toxin, emodin, chrysophanol, and physcion method. METHODS: The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution (4.6 mm × 250 mm, 5 μm) with gradient elution at a flow rate of 1.0 mL · min-1 on a Phenomsil-C18 column with a detection wavelength of 284 nm and a column temperature of 30 ° C. Results: Under the chromatographic conditions, the injection volume of orange cassia seed, aloe emodin, rhein, Huang Caiming, Cassia, emodin, chrysophanol and physcion were 0.10 ~ 0.50 and 0.066 ~ 0.33,0.037 ~ 0.19,0.019 ~ 0.093,0.028 ~ 0.14,0.24 ~ 1.2,0.11 ~ 0.55,0.17 ~ 0.87μg and the chromatographic peak area showed a good linear relationship; sample recovery (n = 6) were 97.0% ~ 102.9%, RSD <2.0%. Conclusion: The method is rapid, accurate and reproducible, which can meet the requirements of the determination of Jiangzhi Ling tablets.