论文部分内容阅读
目的观察砖茶氟在高海拔大鼠体内24 h吸收、分布及排泄的动态变化。方法大鼠分两组,用灌胃方法分别给予氟化钠(NaF)和砖茶水,含氟为300 mg／L,染氟后0．25、0．5、1、2、4、8、16、24 h处死动物,取血清、小肠、股骨、肌肉、心、肝、肺、脾、脑及肾,用氟离子选择电极法测定氟水平。结果两组大鼠骨氟最高,24 h 内呈现明显的蓄积作用,至24 h时,NaF组骨氟增加了约77 mg／kg,砖茶组骨氟增加了约94 mg／kg;血中砖茶氟高峰出现较NaF早,随时间延长,血氟水平逐渐降低,至24 h时,血中NaF和砖茶氟水平仍然很高,可见在 24 h内机体不能将摄入的氟全部排出。两组大鼠各器官不同时间氟水平比较可见,砖茶氟和NaF的代谢趋势基本一致,表现为在大多数器官(脾脏除外)中含氟量峰值均出现在灌胃后0．25 h,然后逐渐下降,至24 h,除骨组织外,两组氟在肌肉、心脏、肝脏、肺脏和肾脏等软组织中也有轻度蓄积作用。结论在高海拔大鼠体内,砖茶氟和NaF的代谢趋势基本一致,不同之处在于砖茶氟在骨组织中水平较高。
Objective To observe the dynamic changes of absorption, distribution and excretion of brick tea fluoride in high altitude rats at 24 h. Methods Rats were divided into two groups. Sodium fluoride (NaF) and brick tea water were respectively administrated by intragastric administration. The contents of fluoride were 300 mg / L, 0.25,0.5,1,2,4,8, The animals were killed at 16 and 24 hours, and the serum, small intestine, femur, muscle, heart, liver, lung, spleen, brain and kidney were collected and fluoride levels were measured by fluoride ion selective electrode method. Results The content of bone fluoride in the two groups was the highest at 24 h. At 24 h, the bone fluoride in the NaF group increased by about 77 mg / kg and that in the brick tea group increased by 94 mg / kg. Fluorine peak appeared earlier than NaF, with the passage of time, blood fluoride levels gradually decreased to 24 h, the blood levels of NaF and brick tea fluorine is still high, we can see within 24 h of the body can not be all the fluoride discharged. Fluorine levels in different organs of the two groups were compared at different time points. The metabolic trends of brick tea fluoride and NaF were basically the same. The peak values of fluoride content in most organs (excluding the spleen) appeared at 0.25 h after gavage Gradually decreased to 24 h, in addition to bone tissue, the two groups of fluoride in muscle, heart, liver, lung and kidney and other soft tissues also have a slight accumulation. Conclusion The metabolic trends of brick tea fluoride and NaF are basically the same in high altitude rats, except that the content of brick tea fluoride is higher in bone tissue.