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碳水化合物含量的测定是植物碳素代谢研究的一项必不可少的分析技术。50年代就有采用蒽酮法测定植物组织可溶性糖含量的报道。由于这种方法对测试仪器、试剂的要求不高,迄今仍得到较为广泛的运用,并有较多报道。我们在工作中观察到影响和干扰该方法的因素较多,测定结果的稳定性差,精度不高。因此,进行了比较系统的摸索。
The determination of carbohydrate content is an indispensable analytical technique for the study of plant carbon metabolism. In the 1950s anthrone method was used to measure the content of soluble sugar in plant tissues. As this method of testing equipment, reagents, less demanding, so far still been more widely used, and more reports. In our work, we observed that there are many factors that affect and interfere with this method. The stability of the measurement results is poor and the accuracy is not high. Therefore, a more systematic exploration.