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本实验应用一氧化氮合成酶抑制剂硝基过氨酸（LNA）和一氧化氮供体SIN－l，探讨NO在常氧和缺氧条件下对肺动脉平滑肌细胞（PASM）增殖的调节。1材料与方法PASM经培养和鉴定“’成功后，选用10代PASM按每孔2万细胞密度接种于96孔培养板，长成层后换含1％透析并灭活的贫血小板血?
In this study, nitric oxide synthase inhibitor nitroprussine (LNA) and nitric oxide donor SIN-l, to explore the NO under normal oxygen and hypoxic conditions on the proliferation of pulmonary artery smooth muscle cells (PASM) regulation. MATERIALS AND METHODS After PASM was cultured and identified, the 10-generation PASM was seeded into 96-well plates at a density of 20,000 cells per well.