论文部分内容阅读
目的评价导流杂交基因芯片(HybriMax)、实时定量PCR(RT-qPCR)在人乳头瘤病毒(HPV)分型和定量分析以及对筛查高级别宫颈上皮内瘤变(CIN)及宫颈癌的临床价值。方法选取2007年5月至2007年10月103例在复旦大学附属妇产科医院宫颈疾病诊疗中心就诊妇女作为研究对象,取宫颈脱落细胞行杂交捕获二代(HCⅡ)、HybriMax及RT-qPCR检测HPV,同时在阴道镜引导下取宫颈活体组织行病理学检查。以HCⅡ检测结果为对照,分析并比较HybriMax及RT-qPCR检测HPV感染与HCⅡ检测结果的一致性;以病理诊断为金标准,分析HybriMax及RT-qPCR对筛查CINⅡ以上病变的灵敏度、阴性预测值。结果 HybriMax、RT-qPCR检测HPV与HCⅡ的符合率分别为86.41%、96.12%;HybriMax筛查CINⅡ以上病变的灵敏度、特异度、阳性预测值、阴性预测值分别为96.30%、50.00%、40.63%、97.44%,RT-qPCR筛查CINⅡ以上病变的灵敏度、特异度、阳性预测值、阴性预测值分别为100%、56.58%、45.00%、100%,且HybriMax、RT-qPCR分别能够将HPV分型和定量。结论导流杂交基因芯片、实时定量PCR检测HPV与HCⅡ比较有较好的一致性,对于CINⅡ以上病变有较高的灵敏度和阴性预测值,且能行HPV分型及定量,可用于宫颈病变的初步筛查。
Objective To evaluate the diagnostic value of HybriMax, real-time quantitative PCR (RT-qPCR) in typing and quantitative analysis of human papillomavirus (HPV) and in the screening of high-grade cervical intraepithelial neoplasia (CIN) and cervical cancer Clinical Value. Methods From May 2007 to October 2007,103 women were selected as the study subjects in the Cervical Disease Treatment Center of Obstetrics and Gynecology Hospital of Fudan University. The second generation (HCⅡ), HybriMax and RT-qPCR HPV, cervical colorectal biopsy under the guidance of pathological examination. HCI test results as a control, analysis and comparison of HybriMax and RT-qPCR detection of HPV infection and HC Ⅱ test results consistency; pathological diagnosis as the gold standard, HybriMax and RT-qPCR screening screening CIN Ⅱ above lesions sensitivity, negative predictive value. Results The sensitivity, specificity, positive predictive value and negative predictive value of HybriMax and RT-qPCR were 86.41% and 96.12%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value were 96.30%, 50.00% and 40.63% , 97.44% respectively. The sensitivity, specificity, positive predictive value and negative predictive value of RT-qPCR in detecting CINⅡ and above lesions were 100%, 56.58%, 45.00% and 100% respectively. HybriMax and RT- Type and quantitative. Conclusion The results of flow-through hybridization (PCR) microarray and real-time quantitative polymerase chain reaction (PCR) showed that HPV and HCⅡhad better agreement and higher sensitivity and negative predictive value for CINⅡ and above, and could be used for genotyping and quantification of HPV. Initial screening.