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AIM:To discuss the expression of glactin-3 in liver metastasis of colon cancer and its inhibition by modified citrus pectin (MCP) in mice.METHODS:Seventy-five Balb/c mice were randomly divided into negative control group (n=15),positive control group (n=15),low MCP concentration group (n=15),middle MCP concentration group (n=15) and high MCP concentration group (n=15).CT26 colon cancer cells were injected into the subcapsule of mouse spleen in positive control group,low,middle and high MCP concentrations groups,except in negative control,to sot up a colon cancer liver metastasis model.The concentration of MCP in drinking water was 0.0%,0.0%,1.0%,2.5% and 5.0% (wt/vol),respectively.Liver metastasis of colon cancer was observed alter 3 wk.Enzyme-linked immunosorbent assay (ELISA) was used to detect the concentration of galectin-3 in serum.Expression of galectin-3 in liver metastasis was detected by immunohistochemistry.RESULTS:Except for the negative group,the percentage of liver metastasis in the other 4 groups was 100%,80%,73.3% and 60%,respectively.The number of liver metastases in high MCP concentration group was significantly less than that in positive control group (P=0.008).Except for the negative group,the median volume of implanted spleen tumor in the other 4 groups was 1.51 cm3,0.93 cm3,0.77 cm3 and 0.70 cm3,respectively.The volume of implanted tumor in middle and high MCP concentration groups was significantly smaller than that in positive control group (P=0.019;P=0.003).The concentration of serum galectin-3 in positive control and MCP treatment groups was significantly higher than that in the negative control group.However,there was no significant difference between them.Except for the negative control group,the expression of galectin-3 in liver metastases of the other 4 groups showed no significant difference.CONCLUSION:Expression of galetin-3 increases significantly in liver metastasis of colon cancer,which can be effectively inhibited by MCP