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目的构建PSGL-1+/-;IL-2-/-小鼠模型。方法将白介素2敲除(IL-2 KO)小鼠与P-选择素糖蛋白配体-1敲除(PSGL-1 KO)小鼠杂交,剪尾提DNA后用PCR法检测小鼠的基因型。结果 IL-2 KO小鼠与PSGL-1 KO小鼠杂交三代后,PSGL-1+/-;IL-2-/-小鼠模型构建成功。结论将IL-2 KO小鼠与PSGL-1 KO小鼠杂交三代后,成功建立了PSGL-1+/-;IL-2-/-小鼠模型。
Objective To construct PSGL-1 +/-; IL-2 - / - mouse model. Methods Interleukin-2 knockout (IL-2 KO) mice were crossed with PSGL-1 knockout (PSGL-1 KO) mice. type. Results The PSGL-1 +/-; IL-2 - / - mouse model was constructed successfully after three generations of IL-2 KO mice and PSGL-1 KO mice. Conclusion The PSGL-1 +/-; IL-2 - / - mouse model was successfully established after three generations of IL-2 KO mice and PSGL-1 KO mice.