将合成的含有随机序列 (NNK) 1 0 的寡核苷酸片段 ,克隆入噬菌体呈现载体噬菌粒 p CANTAB5 E的 Sfi ,N ot 位点 ,即 cp 蛋白信号肽与成熟肽之间 ,电转化 E.coli TG1,构建了噬菌体表面呈现的十肽库 ,实际库容为 3.5 3× 10 7,插入率为 6 6 .7% .经辅助噬菌体 M13KO7超感染后 ,获 The synthesized oligonucleotide fragment containing the random sequence (NNK) 10 was cloned into the Sfi, Not site of the bacteriophage presenting vector phagemid p CANTAB5 E, that is, the cp protein signal peptide was electroporated with the mature peptide E.coli TG1 was constructed to express the decapeptide library on the surface of bacteriophage, the actual storage capacity was 3.5 3 × 10 7 and the insertion rate was 6.6%. After super-infection with helper phage M13KO7,