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目的:探讨DNA甲基转移酶1(DNMT1)基因在喉鳞状细胞癌中的表达及其与临床病理因素的关系。方法:采用免疫组织化学方法检测96例喉鳞状细胞癌中DNMT1基因蛋白的表达,其中66例喉癌组织同时进行实时定量PCR,并分析其DNMT1mRNA的表达与临床病理因素的关系。结果:DNMT1mRNA在喉鳞状细胞癌组织中表达上调(P<0.05)。96例喉癌鳞状上皮细胞中DNMT1蛋白表达全部为阳性(96/96),36%(8/22)癌旁组织的鳞状上皮细胞中DNMT1蛋白表达为阳性,二者差异有统计学意义(P<0.05)。DNMT1mRNA在喉癌组织中表达上调,和患者年龄、性别、临床分期、淋巴结转移情况无关,但与患者吸烟史有显著相关性(P<0.05)。结论:DNMT1可能在喉癌中表达增高从而引起肿瘤的发生,吸烟可能诱导DNMT1的表达增高。
Objective: To investigate the expression of DNA methyltransferase 1 (DNMT1) gene in laryngeal squamous cell carcinoma and its relationship with clinicopathological factors. Methods: The expression of DNMT1 protein in 96 cases of laryngeal squamous cell carcinoma was detected by immunohistochemistry. 66 cases of laryngeal squamous cell carcinoma were detected by real - time quantitative PCR at the same time. The relationship between the expression of DNMT1 mRNA and clinicopathological factors was analyzed. Results: DNMT1 mRNA was up-regulated in laryngeal squamous cell carcinoma (P <0.05). The DNMT1 protein expression was positive in 96 cases (96/96) of squamous cell carcinoma of laryngeal squamous cell carcinoma, and DNMT1 protein was positive in 36% (8/22) squamous cell of paracancerous tissue, the difference was statistically significant (P <0.05). DNMT1 mRNA was up-regulated in laryngeal squamous cell carcinoma, but not related to patient’s age, sex, clinical stage and lymph node metastasis, but had a significant correlation with smoking history (P <0.05). Conclusions: DNMT1 may be up-regulated in laryngeal cancer and cause tumor development. Smoking may induce DNMT1 expression.