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目的 分析中国汉族人口谷胱甘肽S 转移酶 (GSTs)的基因多态性分布。方法 样本为45 0名中国汉族人口 ,采用多重等位基因特异聚合酶链反应 (PCR)方法分析GSTM1和GSTT1基因多态性 ,采用PCR 限制性片段长度多态性方法分析GSTP1+313核苷酸位点的基因多态性。结果 GSTM1缺失型和GSTT1缺失型基因型频率分别为 5 7%和 49% ,同时具有GSTM1缺失型和GSTT1缺失型基因型的个体频率为 2 8 92 % ;而GSTP1+313位点G等位基因频率为 18 7%。并发现该人群中同时具有 3种危险基因型 (GSTM1缺失型、GSTT1缺失型和GSTP1+313A/A)的个体频率为 18 0 4%。GSTs基因型分布不受性别和年龄的影响。结论 中国汉族人口GSTM1、GSTT1和GSTP1基因呈多态性分布 ,其等位基因和基因型频率不同于其他种族
Objective To analyze the genetic polymorphism distribution of glutathione S transferase (GSTs) in Chinese Han population. Methods A sample of 4 0 0 Han Chinese population was used to analyze the polymorphisms of GSTM1 and GSTT1 genes by multiplex allele-specific polymerase chain reaction (PCR). PCR-restriction fragment length polymorphism was used to analyze GSTP1+313 nucleotides. Locus of genetic polymorphisms. Results The frequencies of GSTM1 deletion and GSTT1 deletion genotypes were 57% and 49%, respectively. The frequency of individuals with GSTM1 deletion and GSTT1 deletion genotypes was 28.92%; and the GSTP1+313 locus G allele. The frequency is 187%. It was found that the frequency of individuals with three dangerous genotypes (GSTM1 deletion type, GSTT1 deletion type, and GSTP1+313A/A) was 180.4 %. GSTs genotype distribution is not affected by gender and age. Conclusion The GSTM1, GSTT1 and GSTP1 genes in Chinese Han population are polymorphic and their allele and genotype frequencies are different from those of other ethnic groups.