论文部分内容阅读
采用硅胶和反相C18柱层析方法,首次从瓦宁木层孔菌中分离得到了5个化合物,运用NMR波谱法分析和鉴定为樱花亭、7-甲氧基二氢莰非素、二氢莰非素、4-(3,4-二羟苯基)-3-丁烯-2-酮、hispolon。并通过建立体外二苯基苦味酰基苯肼自由基(·DPPH)、超氧阴离子自由基(·O2-)以及羟自由基(·OH)发生体系,研究了5个化合物对·DPPH、·OH和·O2-的清除作用。结果表明当浓度达到100μg/mL时,化合物4-(3,4-二羟苯基)-3-丁烯-2-酮和hispolon对·DPPH清除率分别为92%和93%,对·OH的清除率分别为90%和95%,而对·O2-的清除率分别为70%和77%,略低于清除·DPPH和·OH的能力;二氢莰非素对·O2-自由基的清除率为39%,强于清除·OH和·DPPH的能力;而樱花亭和7-甲氧基二氢莰非素对3种自由基的清除率均低于30%。2个多酚类化合物清除自由基的能力均强于3个黄酮类化合物。5个化合物清除自由基能力均表现出一定的浓度依赖性。
Five compounds were isolated from Porphyridium cruentum for the first time using silica gel and reverse phase C18 column chromatography. The compounds were identified and characterized by NMR spectroscopy as Sakura Pavilion, 7-methoxydihydantoin, Hydrothymmentin, 4- (3,4-dihydroxyphenyl) -3-buten-2-one, hispolon. In this study, five compounds were tested for DPPH, · OH and DPPH, DPPH, · O2- and hydroxyl radical (· OH) And · O2- scavenging effect. The results showed that the DPPH scavenging rates of the compounds 4- (3,4-dihydroxyphenyl) -3-buten-2-one and hispolon were 92% and 93% respectively at the concentration of 100μg / mL, Of the clearance rate was 90% and 95%, respectively, while · O2- clearance rate was 70% and 77%, slightly lower than the ability to scavenge · DPPH and · OH; Of the clearance rate of 39%, stronger than the ability to scavenge · OH and · DPPH; Sakura Pavilion and 7-methoxy Dihydrogudin on three kinds of free radicals were less than 30% clearance. The ability of 2 polyphenols to scavenge free radicals was stronger than that of 3 flavonoids. Five compounds scavenged free-radical ability showed a certain concentration-dependent.