【摘 要】
：
The autonomic nervous system contributes to prostate cancer proliferation and metastasis.However,the exact molecular mechanism remains unclear.In this study,muscarinic acetylcholine receptor M 1 (CHRM 1) expression was measured via immunohistochemical ana
【机 构】
：
Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, China
论文部分内容阅读
The autonomic nervous system contributes to prostate cancer proliferation and metastasis.However,the exact molecular mechanism remains unclear.In this study,muscarinic acetylcholine receptor M 1 (CHRM 1) expression was measured via immunohistochemical analysis in human prostate cancer tissue array slides.PC-3,LNCaP,and A549 cells were treated with pirenzepine or carbachol,and the cell migration and invasion abilities were evaluated.Western blotting and quantitative real-time PCR were performed to measure GLI family zinc finger 1 (GLI1),patched 1 (PTCH1),and sonic hedgehog (SHH) expression levels.High expression of CHRM1 was found in early-stage human prostate cancer tissues.In addition,the selective CHRM1 antagonist pirenzepine inhibited PC-3,LNCaP,and A549 cell migration and invasion,but the agonist carbachol promoted the migration and invasion of these three cell lines.Muscarinic signaling can be relayed by hedgehog signaling.These data show that CHRM1 is involved in the regulation of prostate cancer migration and invasion through the hedgehog signaling pathway.
其他文献
The TOPARP study showed the beneficial utility of olaparib in a heavily pretreated population of metastatic castration-resistant prostate cancer who were biomarker-positive for aberrations in DNA repair gene.A higher response rate to olaparib of 88％ (14 o
John Michael Bedford (Figure 1) studied at Sidney Sussex College,Cambridge University (1952-1958),and gained his BA (1955) andMA (1958) in Natural Sciences,with postgraduate training in Veterinary Medicine and Surgery (Vet MB 1958).He had academic appoint
Dear Editor,rnProstatic calcification is a common condition observed by the urology community.The earliest description was proposed by Donatus in 1586,followed by Pohl in 1737.1 In clinical practice,an overwhelming majority of prostatic calcification case
Speckle-type POZ (pox virus and zinc finger protein) protein (SPOP) is an E3 ubiquitin ligase adaptor protein that specifically promotes the nbiquitination and proteasome degradation of proteins.SPOP mutations are frequent in prostate cancer,and in a prev
Men with infertility are subjected to numerous diagnostic tests in attempts to determine the nature of their condition.Initially,medical,surgical,and birth histories are elicited with investigations including semen analyses,hormonal panels,and imaging stu