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为了解湖北土家族人群X-STR基因座的多态性,本文探讨其在法医学上的应用价值,抽选了210人湖北土家族无关个体样本,采用PCR扩增和末端标记荧光循环测序的方法,分析其DXS6804、DXS7130、DXS7132和DXS8378 4个X-STR基因座的等位基因频率及基因型分布。结果表明:其中的五项法医学应用指标与其他民族及种族存在显著区别,差异有统计学意义(P<0.05)。这一研究结果证实湖北土家族群体中DXS6804、DXS7130、DXS7132和DXS8378 4个X-STR基因座具有较高遗传多态性,在法医学个体识别和亲权鉴定中有较高应用价值,同时也丰富了少数民族STR基因数据库。
In order to understand the polymorphism of X-STR locus in Tujia nationality in Hubei province, the paper explores its value in forensic science. A total of 210 native Tujia unrelated individuals in Hubei Province were selected. PCR amplification and end-labeled fluorescent cycle sequencing The allele frequency and genotype distribution of four X-STR loci of DXS6804, DXS7130, DXS7132 and DXS8378 were analyzed. The results showed that the five forensic application indexes were significantly different from those of other ethnic groups and ethnic groups (P <0.05). The results of this study confirm that the four X-STR loci of DXS6804, DXS7130, DXS7132 and DXS8378 in Hubei Tujia population have higher genetic polymorphisms, which have higher value in forensic identification and paternity testing and are also enriched Minority STR gene database.