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采用RAPD-SCAR分子标记技术,从300条RAPD随机引物中筛选到了对条形柄锈菌Puccinia striiformis f.sp.tritici33号生理小种特异的2条引物,将特异性片段回收、克隆和测序后(Gen Bank注册号为AB914691和AB914692),依据其序列设计出了2对引物S261F33/S261R33和S300F33/S300R33,能够特异性地从33号生理小种基因组DNA及发病小麦叶片总DNA中分别扩增出247bp和763bp的片段,其结果与采用常规的鉴别寄主法鉴定的结果一致。因此,这2对引物都可用于条形柄锈菌33号生理小种的快速鉴定与监测。
Two primers specific to Puccinia striiformis f. Sp. Tritici33 race were screened from 300 RAPD random primers using RAPD-SCAR molecular markers. The specific fragments were recovered, cloned and sequenced (GenBank accession numbers AB914691 and AB914692). Two pairs of primers, S261F33 / S261R33 and S300F33 / S300R33, were designed according to their sequences and were able to amplify specifically from genomic DNA of 33 # Fragments of 247 bp and 763 bp were obtained, the results of which are consistent with those identified by conventional discriminating host methods. Therefore, these two pairs of primers can be used for the rapid identification and monitoring of P. rapae 33 race.