论文部分内容阅读
[目的]探讨在二甲基亚硝胺(DMN)诱导小鼠肝损伤模型中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的动态表达。[方法]以DMN15 mg/kg腹腔注射小鼠,对照组小鼠腹腔注射0.85%氯化钠溶液,酶联免疫吸附法检测各时间点血清TNF-α、IL-1β水平;定量PCR法检测各时间点肝组织TNF-α、IL-1βmRNA的表达;免疫组化法检测各时间点肝组织TNF-α、IL-1β的蛋白表达。[结果]DMN造模后,TNF-α及IL-1β基因和蛋白表达在各个时间点都有一定程度的上升,而且均在造模后48h时达到高峰。[结论]在DMN诱导的小鼠肝损伤模型中TNF-α及IL-1β显著升高,提示炎症坏死可能是DMN诱导小鼠肝损伤的主要机制。
[Objective] To investigate the dynamic expression of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in a mouse model induced by DMN. [Method] The mice in the control group were intraperitoneally injected with 0.85% sodium chloride solution with DMN15 mg / kg and the levels of TNF-α and IL-1β in the serum were detected by enzyme-linked immunosorbent assay (ELISA) The expression of TNF-α and IL-1βmRNA in liver tissue were detected by immunohistochemistry. The protein expressions of TNF-α and IL-1β in liver tissues were detected by immunohistochemistry. [Results] The expression of TNF-α and IL-1β gene and protein increased to some extent at each time point after DMN modeling, and all reached the peak 48h after modeling. [Conclusion] The levels of TNF-α and IL-1β in DMN-induced mouse liver injury model are significantly increased, suggesting that inflammation and necrosis may be the main mechanism of DMN-induced liver injury in mice.