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以Ti O_2纳米颗粒光催化反应为模型,研究了反应过程中的活性氧(ROS)产生以及活性氧淬灭的反应动力学模型。对苯二甲酸分子与体系中的光催化反应产生的OH·反应,生成具有荧光性质的2-羟基对苯二甲酸(λ_(ex)=315 nm,λ_(em)=425 nm),因此对苯二甲酸作为氧化探针分子与体系中的生物抗氧化剂(AOs)分子竞争与ROS的反应,根据体系的荧光、反应时间以及AOs的浓度建立了AOs淬灭ROS的反应动力学模型。根据此模型推导AOs清除ROS的动力学常数,发现常见的生物抗氧化剂的抗氧化活性大小顺序为:硫辛酸、没食子酸、谷胱甘肽、尿酸、维生素C、维生素E、水溶性维生素E和胆红素。
The reaction kinetics model of reactive oxygen species (ROS) generation and reactive oxygen species quenching was studied with the photocatalytic reaction of Ti O 2 nanoparticles as a model. The terephthalic acid molecule reacted with OH · generated by the photocatalytic reaction in the system to generate 2-hydroxyterephthalic acid (λex = 315 nm, λ = (em) = 425 nm) Phthalic acid as an oxidative probe molecule competes with biological antioxidant (AOS) molecules in the system for the reaction with ROS. According to the fluorescence and reaction time of the system and the concentration of AOs, the reaction kinetics model of AOs for quenching ROS was established. According to this model, the kinetic constants of ROS removal from AOs were deduced. The antioxidant activity of common biological antioxidants was found to be in the order of lipoic acid, gallic acid, glutathione, uric acid, vitamin C, vitamin E, water-soluble vitamin E and Bilirubin.