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目的通过检测S100B蛋白、脑源性神经营养因子(BDNF)、胰岛素样生长因子-1(IGF-1)在癫痫婴儿血清中的水平,探讨其与婴儿癫痫发作的相关性。方法选取2012年1月至2016年12月收治的88例发作性疾病婴儿为研究对象,按发作类型分为48例癫痫发作组及40例非癫痫性发作组。酶联免疫吸附测定(ELISA)法测定婴儿发作性表现后4、24 h血清S100B蛋白、BDNF、IGF-1水平。另外选择40例同期体检健康婴儿作为对照。比较三组对象及癫痫发作患儿发作后不同时间(4、24 h)、不同发作持续时间(抽搐时间>5 min 21例和抽搐时间≤5 min 27例)、不同发作类型(全面性发作22例,局限性发作26例)血清S100B蛋白、BDNF、IGF-1水平的差异,分析其与癫痫发作的相关性。结果发作后24 h血清S100B蛋白、BDNF、IGF-1水平比较,呈癫痫发作组>非癫痫发作组>对照组(P均<0.01);癫痫组患儿发作后24 h血清三指标水平明显低于发作后4 h(P均<0.01)。抽搐时间>5 min患儿血清S100B蛋白、BDNF、IGF-1水平均高于抽搐时间≤5 min患儿(P均<0.01);血清S100B蛋白水平全面性发作患儿明显高于局限性发作患儿(P<0.01),血清BDNF、IGF-1水平全面性发作与局限性发作患儿比较差异无统计学意义(P均>0.05)。结论血清S100B蛋白、BDNF、IGF-1水平检测有助于癫痫发作的临床诊断,可作为癫痫患儿神经损伤程度评估的参考指标。
Objective To investigate the relationship between S100B protein, brain-derived neurotrophic factor (BDNF) and insulin-like growth factor-1 (IGF-1) in the serum of epileptic infants to explore their relationship with seizures in infants. Methods Eighty-eight infants with seizures were selected from January 2012 to December 2016. The seizures were divided into 48 epileptic seizures and 40 non-epileptic seizures. Serum levels of S100B, BDNF and IGF-1 at 4 and 24 h after the onset of infantile seizures were measured by enzyme-linked immunosorbent assay (ELISA). In addition, 40 cases of healthy infants were selected as controls during the same period. The duration of different episodes (21 cases of convulsions> 5 minutes and 27 cases of convulsions ≤ 5 minutes) were compared between the three groups at different times (4,24 hours) after onset of seizures, and the different types of attacks Cases, limitations of attack in 26 cases) serum S100B protein, BDNF, IGF-1 levels were analyzed, and its correlation with seizures. Results Serum levels of S100B, BDNF and IGF-1 at 24 h after onset were significantly higher than those in seizure group> non-seizure group> control group (all P <0.01). Serum levels of three indicators of seizure group were significantly lower at 24 h 4 h after the onset (all P <0.01). The levels of serum S100B protein, BDNF and IGF-1 in children with convulsions> 5 min were significantly higher than those in children with convulsions ≤ 5 min (all P <0.01); children with S100B protein level seizures were significantly higher than those with limited seizures (P <0.01). There was no significant difference in the serum levels of BDNF and IGF-1 between children with full-blown and localized seizures (all P> 0.05). Conclusion Serum levels of S100B, BDNF and IGF-1 may contribute to the clinical diagnosis of seizures and may be used as a reference index for assessing the degree of nerve injury in children with epilepsy.