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采用逆转录- 聚合酶链式反应检测了慢性足底电击结合噪声应激致高血压大鼠下丘脑、延髓、中脑、垂体和肾上腺等组织中编码肾上腺髓质素的肾上腺髓质素前肽原(preproadrenomedullin, ppADM) 基因以及ADM 的特异性受体组件降钙素受体样受体(calcitonin-receptor-like receptor,CRLR)和受体活性调节蛋白2 和3(receptor-activity-modifying proteins, RAMP2 和RAMP3)表达的变化。我们观察到:与对照组相比,以 3- 磷酸甘油醛脱氢酶作为内参照,15 d 足底电击结合噪声应激引起下丘脑、垂体和肾上腺中ppADM mRNA表达上调,而在延髓和中脑表达明显下调(P<0.01 或 P<0.05); CRLR基因表达量正常时在下丘脑相对较高,应激15 d 后CRLR 表达在延髓、中脑和下丘脑下调(P<0.01 或 P<0.05), 而在垂体和肾上腺的表达无明显变化;应激后RAMP2 基因在延髓和下丘脑表达上调,而在肾上腺表达显著下调(P <0.01), 其他部位无明显变化;RAMP3 基因在对照组大鼠的中脑和下丘脑表达较高,在应激性高血压大鼠的下丘脑和垂体表达上调(P<0.01 或P<0.05), 而在中脑和肾上腺表达下调(P<0.05), 在延髓中的表达变化无统计学差异。上述结果提示:慢性足底电击结合噪声应激引起明显的中枢和下丘脑- 垂体-肾上腺轴ADM 及其受体组件CRLR/RAMP2 或CRLR/R
Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of adrenomedullin-derived adrenomedullin propeptide in the tissues of hypothalamus, medulla oblongata, midbrain, pituitary and adrenal of chronic stress-induced rat model of stress-induced hypertension (PPADM) gene and calcitonin receptor-like receptor (CRLR) and receptor-activity-modifying proteins RAMP2 and RAMP3) expression changes. We observed that ppADM mRNA was up-regulated in the hypothalamus, pituitary and adrenal gland by 15-day foot-shock combined with noise-induced stress compared with control group using glyceraldehyde-3-phosphate dehydrogenase as internal control. (P <0.01 or P <0.05). CRLR gene expression was relatively high in the hypothalamus and CRLR expression was down-regulated in medulla, midbrain and hypothalamus after 15 d of stress (P <0.01 or P <0.05) ), While there was no significant change in pituitary and adrenal. RAMP2 gene was up-regulated in medulla oblongata and hypothalamus but down-regulated in adrenal gland (P <0.01) but not in other sites. The expression of RAMP3 in control group The rats had higher expression of midbrain and hypothalamus, upregulated in hypothalamus and pituitary (P <0.01 or P <0.05), but down-regulated in midbrain and adrenal (P <0.05) There was no significant difference in the expression in the medulla. The above results suggest that chronic plantar shock combined with noise stress causes significant CNS and hypothalamic-pituitary-adrenal axis ADM and its receptor components CRLR / RAMP2 or CRLR / R