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目的探讨ABCG5和ABCG8与结石形成的关系。方法测定结石组病人结石胆固醇含量,确定为胆固醇结石。比较禁食情况下胆固醇结石病人和胃癌病人中血脂、脂蛋白的差异,采用实时PCR方法比较胆固醇结石病人与胃癌病人ABCG5和ABCG8mRNA的差异。结果两组病例血脂、脂蛋白比较无明显差异。胆固醇结石组ABCG5/beta-actin和ABCG8/beta-actin均高于胃癌组(为17.6%±9.8%vs13.5%±6.2%,P<0.01;18.9%±7.1%vs16.5%±4.2%,P<0.01),说明胆固醇结石病人在基础情况下ABCG5和ABCG8有较高的转录水平。结论本研究提示胆固醇结石的形成与肝脏ABCG5和ABCG8高表达,向胆汁中转运较多的胆固醇有关。
Objective To investigate the relationship between ABCG5 and ABCG8 and stone formation. Methods The stones in the calculus group were determined for the cholesterol content of the calculus, which was determined as cholesterol gallstone. To compare the differences of lipids and lipoproteins in cholesterol gallstone patients and gastric cancer patients under fasting conditions, and to compare the differences of ABCG5 and ABCG8 mRNA between patients with cholesterol gallstone and gastric cancer by real-time PCR. Results There was no significant difference in serum lipids and lipoproteins between the two groups. The levels of ABCG5 / beta-actin and ABCG8 / beta-actin in cholesterol group were significantly higher than those in gastric cancer group (17.6% ± 9.8% vs13.5% ± 6.2%, P <0.01; 18.9% ± 7.1% vs16.5% ± 4.2% , P <0.01), indicating that cholesterol gallstone patients in the basic case of ABCG5 and ABCG8 have a higher transcription level. Conclusions This study suggests that the formation of cholesterol stones is associated with the high expression of ABCG5 and ABCG8 in the liver and more cholesterol in the bile.