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目的了解惠州市桶装饮用天然矿泉水和包装饮用水中铜绿假单胞菌污染状况,为桶装饮用水卫生监督管理和相关水源性疾病的预防及控制提供科学依据。方法采用国标《GB/T 8538-2008饮用天然矿泉水检验方法》,对2013-2015年桶装饮用天然矿泉水和包装饮用水进行铜绿假单胞菌检测并对检出菌株进行药敏试验。结果 3年间223份桶装饮用水中有24份样品检出铜绿假单胞菌,总阳性率为10.76%;各类型桶装水均有检出,饮用天然矿泉水、纯净水、山泉水及其他饮用水的阳性率分别为12.86%、9.28%、9.76%和13.33%;24株典型菌株中,17株产绿脓菌素,6株产荧光色素,1株产脓红素;铜绿假单胞菌检出与大肠菌群检验结果无显著相关性;药敏试验结果显示24株阳性菌株对哌拉西林、头孢吡肟等11种抗生素均无耐药性,对氨苄西林、头孢唑啉等7种抗生素耐药,耐药率100.00%。结论惠州市桶装饮用天然矿泉水和包装饮用水中均存在铜绿假单胞菌污染风险隐患,阳性样品中存在3种典型菌株的菌落形态,建议桶装饮用水生产企业采取控制措施,相关监督部门应加强监督管理。
Objective To understand the pollution status of Pseudomonas aeruginosa in bottled natural mineral water and packaged drinking water in Huizhou and provide a scientific basis for the prevention and control of bottled drinking water health supervision and management and related waterborne diseases. Methods The national standard “GB / T 8538-2008 Drinking Natural Mineral Water Test Method” was used to detect Pseudomonas aeruginosa from 2013 to 2015 bottled drinking natural mineral water and packaged drinking water. The susceptibility test of the tested strains was carried out. Results Among the 223 bottled drinking water samples, Pseudomonas aeruginosa was detected in 24 samples with a total positive rate of 10.76% over the three years. All types of bottled water were detected and drinking natural mineral water, pure water, mountain spring water, The positive rates of other drinking water were 12.86%, 9.28%, 9.76% and 13.33% respectively. Of the 24 typical strains, 17 were pyocyanin, 6 were fluorescent and 1 was pyocyanin. There was no significant correlation between the detection of coliform bacteria and the results of coliform test. The results of drug sensitivity test showed that 24 strains were not resistant to 11 antibiotics, such as piperacillin and cefepime. For ampicillin and cefazolin Seven kinds of antibiotic resistance, drug resistance rate of 100.00%. Conclusion There is a potential risk of Pseudomonas aeruginosa contamination in bottled drinking natural mineral water and packaged drinking water in Huizhou City. There are three typical strains of colony morphology in the positive samples. It is suggested that bottled drinking water producers adopt control measures and relevant supervision Departments should strengthen supervision and management.