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为探讨Wnt信号通路在孤独症发病中的作用,我们检测了Wnt信号通路中的两个关键信号蛋白分子β-catenin和GSK-3β在不同年龄阶段孤独症模型大鼠前额叶皮层,海马及小脑中的表达,同时对小脑进行了GSK-3β免疫组织化学染色检测。结果显示:孤独症模型动物的上述三个关键脑区内,β-catenin的表达水平显著升高,而GSK-3β的表达水平则明显降低;小脑内GSK-3β免疫反应阳性Purkinje细胞也明显减少。这些结果表明,孤独症模型大鼠脑内的Wnt信号通路信号传导亢进,而亢进的结果可能正是导致已知的孤独症脑内神经元发育异常的原因之一。由此提示:Wnt信号通路在孤独症的发病中起重要作用。
To investigate the role of Wnt signaling in the pathogenesis of autism, we examined the effects of two key signaling proteins, β-catenin and GSK-3β, on Wnt signaling pathway in the prefrontal cortex, hippocampus and cerebellum The expression of GSK-3β in the cerebellum was detected by immunohistochemistry. The results showed that the expression ofβ-catenin was significantly increased and the expression of GSK-3β was significantly decreased in the three key brain regions of autistic model animals, and the number of GSK-3β immunoreactive Purkinje cells in cerebellum was significantly decreased . These results indicate that the Wnt signaling pathway in the autistic rat brain is hyperactive, and that the result of hyperactivity may be one of the causes of neuronal dysplasia in the brain of autism. This suggests that: Wnt signaling pathway plays an important role in the pathogenesis of autism.