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目的 :研究IL 6对大鼠脑缺血再灌注 (CIRF)后海马及纹状体的NOS和Fos阳性神经元表达的作用。方法 :采用大脑中动脉栓塞法 (MCAO)对 2 4只Wistar大鼠左侧大脑中动脉构成CIRF模型的基础上分两组 :即单纯CIRF对照组 (n =8) ;经同侧脑室事先注入IL 6的实验组 (n =16)。分别进行NADPH脱氢酶反应显示NOS和免疫组织化学染色显示Fos表达。观察了CIRF后 1、2、4、6小时海马及纹状体处NOS和Fos阳性神经元数量的变化 ,以及IL 6对其变化的影响。结果 :大鼠CIRF后纹状体缺血中心区缺少NOS和Fos的表达 ,而纹状体周围区NOS和Fos阳性神经元明显增加 ;海马各部NOS和Fos阳性神经元均明显增加 ,其中CA1区增加最显著。但预先经侧脑室注射IL 6的实验组中 ,大鼠海马及纹状体NOS和Fos阳性神经元明显低于对照组。IL 6明显抑制了NOS及Fos表达。结论 :提示IL 6可能参与脑缺血性神经元损伤的调控作用
Objective: To investigate the effect of IL 6 on NOS and Fos positive neurons in hippocampus and striatum after cerebral ischemia-reperfusion (CIRF) in rats. Methods: The left middle cerebral artery of 24 Wistar rats were divided into two groups based on the CIRF model by middle cerebral artery occlusion (MCAO). The CIRF control group (n = 8) Experimental group of IL6 (n = 16). Respectively NADPH dehydrogenase reaction showed that NOS and immunohistochemical staining showed Fos expression. The changes of NOS and Fos positive neurons in the hippocampus and striatum at 1, 2, 4 and 6 hours after CIRF were observed, and the effect of IL 6 on the changes was also observed. Results: The expression of NOS and Fos in the central ischemic area of the striatum were absent in CIRF rats, while the number of NOS and Fos positive neurons in the peri-striatum area was significantly increased. The NOS and Fos positive neurons in hippocampus were significantly increased, Increase the most significant. However, in experimental groups injected with IL 6 into the lateral ventricle, the NOS and Fos positive neurons in the hippocampus and striatum were significantly lower than those in the control group. IL 6 significantly inhibited NOS and Fos expression. Conclusion: IL 6 may be involved in the regulation of cerebral ischemic neuronal injury