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建立一种光纤化学传感同步测定吸收光谱和荧光光谱的新方法。自制同步吸收-荧光比色皿,结合光纤化学传感技术,建立同步吸收-荧光光谱法检测仪器,分别测定罗丹明B,维生素B2,维生素B6等溶液的同步吸收-荧光光谱,且与传统的紫外-可见吸光光度法和荧光法进行对比。同步吸收-荧光光谱法测得罗丹明B,维生素B2,维生素B6与传统吸光光度法和荧光光谱法检测的吸收光谱图和荧光光谱的图谱大体一致。最大荧光强度波长与传统的荧光方法比较准确度高,但最大吸收波长略有偏差。同步吸收-荧光光谱法等同于同步测定荧光物质的吸收光谱和荧光光谱,实现二光谱合二为一,测定最大发射波长时准确度高,但最大吸收波长略有偏差,值得深一步研究。
A new method for simultaneous determination of absorption and fluorescence spectra by optical fiber chemical sensing was established. Homegrown synchronous absorption-fluorescence cuvettes, combined with optical fiber chemical sensing technology, the simultaneous absorption-fluorescence spectrometry detection apparatus was established to measure the simultaneous absorption-fluorescence spectra of rhodamine B, vitamin B2, vitamin B6 and other solutions, UV-visible spectrophotometry and fluorescence comparison. Synchronous absorption - fluorescence spectroscopy measured rhodamine B, vitamin B2, vitamin B6 and conventional absorption spectrophotometry and fluorescence spectroscopy to detect absorption spectra and fluorescence spectra are generally the same. The maximum fluorescence intensity wavelength is more accurate than the traditional fluorescence method, but the maximum absorption wavelength is slightly deviated. Simultaneous absorption-fluorescence spectrometry is equivalent to the simultaneous determination of the absorption and fluorescence spectra of the fluorescent material, which achieves the combination of the two spectra. The determination of the maximum emission wavelength is of high accuracy, but the maximum absorption wavelength is slightly deviated, which is worth further study.