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以Fe3O4磁性纳米粒子为载体、多巴胺(DA)为功能单体、血红蛋白(Hb)为模板分子,用氯铂酸氧化DA生成聚多巴胺(PDA),同时氯铂酸还原为铂纳米粒子(Pt NPs),与Hb一起负载于Fe3O4纳米粒子表面,洗脱Hb后合成了表面分子印迹磁性纳米粒子(印迹Fe3O4/PDA-Pt NPs).将印迹Fe3O4/PDA-Pt NPs修饰于磁性玻碳基底表面,制得印迹Fe3O4/PDA-Pt NPs修饰电极.实验结果表明,印迹Fe3O4/PDA-Pt NPs具有良好的水溶性,粒径分布均匀,生成的Pt NPs具有良好的导电性和刚性.用印迹Fe3O4/PDA-Pt NPs构建的传感器对Hb具有良好的识别性,在0.14~2.7μg·m L-1Hb浓度范围与交流阻抗变化值呈良好的线性关系,检出限(S/N=3)为0.05μg·m L-1.
Fe3O4 magnetic nanoparticle as a carrier, dopamine (DA) as a functional monomer, hemoglobin (Hb) as a template molecule, oxidation of DA with chloroplatinic acid to generate polydopamine (PDA), while the reduction of platinum to platinum nanoparticles ) And Hb were loaded on the surface of Fe3O4 nanoparticles, the surface molecular imprinted magnetic nanoparticles (imprinted Fe3O4 / PDA-Pt NPs) were synthesized after the Hb was eluted.The imprinted Fe3O4 / PDA-Pt NPs were modified on the surface of magnetic glassy carbon substrate, The experimental results show that the imprinted Fe3O4 / PDA-Pt NPs have good water-solubility and uniform particle size distribution, and the Pt NPs produced have good electrical conductivity and rigidity.The imprinted Fe3O4 / The sensor constructed by PDA-Pt NPs has a good recognition of Hb, and has a good linear relationship with the change of AC impedance in the concentration range of 0.14-2.7μg · m L-1Hb. The detection limit (S / N = 3) is 0.05 μg · m L-1.