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目的:测定雌激素受体在人涎腺粘液表皮样癌细胞系Mec-1以及高转移株Mc-3上的表达,为口腔肿瘤临床内分泌治疗提供依据。方法:用免疫细胞化学方法和RT-PCR方法检测雌激素受体在人涎腺粘液表皮样癌细胞上的表达,并对其表达量进行相对定量分析。结果:经免疫细胞化学和RT-PCR方法可检测到ER在人涎腺粘液表皮样癌细胞上有表达,并且Mc3细胞系ER表达强于MEC-1;两种细胞系ER-α表达均略强于ER-β。结论:雌激素有可能通过它的受体对人粘液表皮样癌细胞产生一定的影响。ERα/ERβ比率升高在粘液表皮样癌发生过程中可能起一定作用,ER的表达升高可能与肿瘤转移力有关。
OBJECTIVE: To determine the expression of estrogen receptor in human salivary gland mucoepidermoid carcinoma cell line Mec-1 and its highly metastatic line Mc-3, and to provide basis for clinical endocrine therapy of oral tumors. Methods: The expression of estrogen receptor in mucoepidermoid carcinoma cells of human salivary glands was detected by immunocytochemistry and RT-PCR, and the relative quantitative expression of estrogen receptor was analyzed. Results: The expression of ER in human salivary gland mucoepidermoid carcinoma cells was detected by immunocytochemistry and RT-PCR, and the expression of ER in Mc3 cells was stronger than that in MEC-1. The expression of ER-α in both cell lines was slightly Stronger than ER-β. Conclusion: It is possible that estrogen exerts its influence on human mucoepidermoid carcinoma cells through its receptor. The increased ERα / ERβ ratio may play a role in the development of mucoepidermoid carcinoma. The increased expression of ER may be related to tumor metastasis.