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目的 探讨煤尘致肺泡巨噬细胞 (alveolar macrophages,AM) ,损伤过程中是否存在着氧化损伤机制。 方法 应用细胞化学法显示煤尘作用于 AM产生的 O·2 和 H2 O2 、并做定量测定及相关分析 ,以 Mg2 + - AT-Pase组织化学染色作为检测膜变化的指标。 结果 煤尘致 AM产生的氧自由基较矽尘的少 ,同时细胞的形态结构和膜酶的损伤程度也较低。 结论 (1)应用抗氧化剂可减弱煤尘对 AM所致的损伤 ,进而预防肺成纤维细胞增殖 ;(2 )与矽尘相比 ,煤尘致 AM氧化损伤的程度较弱 ;(3)形态学方法定位显示自由基较之自由基总体测定技术更能直观、具体地阐明细胞损伤的实际状况。
Objective To investigate the mechanism of oxidative damage in alveolar macrophages (AM) induced by coal dust. Methods O 2 and H 2 O 2 generated by the action of coal dust on AM were detected by cytochemistry. Quantitative analysis and correlation analysis were performed. The change of Mg 2 + - AT-Pase histochemical staining was used as an index to detect membrane changes. As a result, AM produced less oxygen free radicals than silica dust, and at the same time, the cell morphological structure and membrane enzyme damage were also lower. Conclusions (1) The application of antioxidants can reduce the damage caused by coal dust to AM, and then prevent the proliferation of lung fibroblasts. (2) Compared with silica dust, the extent of AM oxidative damage caused by coal dust is weak. (3) Learning methodological positioning shows that free radicals are more intuitive and specific than free radical total determination techniques to clarify the actual condition of cell injury.