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目的：观察海风藤对犬脑干缺血兴奋性氨基酸含量的影响及对其缺血损伤的保护作用。方法：建立犬脑干缺血模型，观察并比较生理盐水（ＮＳ）对照组及海风藤用药组脑干缺血后各时点兴奋性氨基酸（ｅｘｃｉｔａｔｏｒｙａｍｉｎｏａｃｉｄｓ，ＥＡＡｓ）含量及神经元病理学损伤的差异。结果：ＮＳ组缺血０．５ｈ，ＥＡＡｓ含量轻度升高（Ｐ＞０．０５），神经元呈现轻度缺血改变。缺血３ｈ，ＥＡＡｓ含量显著升高（Ｐ＜０．０５），神经元呈现中度缺血改变。缺血６～１２ｈ，ＥＥＡｓ含量进一步增加（Ｐ＜０．０１），神经元严重受损，并出现坏死。在缺血前应用海风藤后，与ＮＳ组相比，缺血０．５ｈ时，缺血脑干ＥＡＡｓ含量轻度下降（Ｐ＞０．０５），神经元光电镜下无缺血性损伤表现。缺血３～６ｈ，ＥＡＡｓ含量明显下降（Ｐ＜０．０５），神经元呈现中度缺血改变。在缺血１２ｈ，ＥＡＡｓ含量降低仍显著（Ｐ＜０．０５），神经元病理学表现为重度缺血损伤。结论：海风藤对脑干缺血损伤具有明显保护作用，这种保护作用与海风藤可降低脑干缺血区ＥＡＡｓ含量有关。
OBJECTIVE: To observe the effect of Kadsura bungeana on the content of ischemic excitatory amino acids in canine brain stem and its protective effect on ischemic injury. METHODS: A canine model of brain stem ischemia was established. The levels of excitatory amino acid (EAAs) and neuronal pathological lesions at various time points after brainstem ischemia in the normal saline (NS) control group and the seaward cannabis herb group were observed and compared. . RESULTS: In the NS group, the EAAs content was slightly increased (P>0.05) after ischemia for 0.5 h, and the neurons showed mild ischemic changes. At 3h after ischemia, EAAs content was significantly increased (P<0.05), and neurons exhibited moderate ischemic changes. After 6 to 12 hours of ischemia, EAEs content increased further (P<0.01), neurons were severely damaged, and necrosis appeared. After applying isoflavone before ischemia, compared with NS group, EAAs content in ischemic brainstem decreased slightly after ischemia for 0.5 h (P>0.05), and there was no ischemic injury under neuron microscopy. . After 3 to 6 hours of ischemia, the content of EAAs decreased significantly (P<0.05), and neurons showed moderate changes in ischemia. At 12h after ischemia, EAAs content was still significantly lower (P<0.05), neuronal pathology showed severe ischemic injury. Conclusion: Houttuynia had a protective effect on brain stem ischemic injury. This protective effect is related to the decrease of EAAs content in brain stem ischemia area.