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为探究南方人工林生态系统土壤酶活性对硫、氮复合沉降的响应机制,本研究采用二元二次正交回归旋转设计方法,设置16个硫、氮复合沉降处理组和1个空白对照组,研究1 a硫、氮复合沉降下邓恩桉人工幼龄林土壤上层(0~20 cm)和下层(20~40 cm)的酸性磷酸酶、脲酶、蔗糖酶和过氧化氢酶活性与硫、氮复合沉降之间关系,并建立回归模型。对17组数据进行单因素方差分析(one-way ANOVA),结果显示除上、下层过氧化氢酶活性组间差异均不显著外(p>0.05),酸性磷酸酶、脲酶和蔗糖酶活性组间均存在差异显著的土壤分层(p<0.05)。利用土壤分层中差异显著的酸性磷酸酶、脲酶和蔗糖酶活性进行分析、建模,结果表明:酸性磷酸酶仅对氮沉降的独立效应响应显著,与空白对照组相比,氮沉降抑制酸性磷酸酶活性;脲酶仅对硫沉降和氮沉降的独立效应响应显著,且脲酶活性在硫、氮沉降下降低,以氮沉降抑制作用最强;蔗糖酶对硫、氮沉降的独立效应和交互作用均响应显著,表现出硫、氮沉降抑制蔗糖酶活性的特征。研究表明,1 a硫、氮沉降抑制邓恩桉人工幼龄林红壤表层(0~40 cm)的土壤酶活性,引起土壤肥力降低。
In order to explore the response mechanism of soil enzyme activities to sulfur and nitrogen deposition in the plantation ecosystems in southern China, a binary quadratic orthogonal rotation design method was used in this study. Sixteen sulfur and nitrogen composite sedimentation treatment groups and one blank control group , The activities of acid phosphatase, urease, sucrase and catalase in the top soil layer (0-20 cm) and the lower layer (20-40 cm) Compound settlement between the relationship and establish a regression model. One-way ANOVA was used to analyze the data of 17 groups. The results showed that there was no significant difference except the upper and lower catalase activity between the two groups (p> 0.05), acid phosphatase, urease and invertase activity group There was significant difference in soil stratification (p <0.05). The acid phosphatase, urease and sucrase activities were analyzed and modeled with significant differences in soil stratification. The results showed that acid phosphatase only had significant response to the independent effect of nitrogen deposition. Compared with the control group, nitrogen deposition inhibited the acidity The urease only had a significant response to the independent effects of sulfur deposition and nitrogen deposition. The urease activity decreased with the deposition of sulfur and nitrogen, and the strongest inhibition was nitrogen deposition. The independent effect and interaction of sucrase on sulfur and nitrogen deposition All showed significant response, which showed that sulfur and nitrogen deposition inhibited the activity of sucrase. The results show that 1 a sulfur and nitrogen deposition can inhibit soil enzyme activities of soil surface layer (0-40 cm) in Eucalyptus dunnii young plantations, and decrease soil fertility.