Purpose: To investigate the intraocular growth and biological characteristics of mice embryonic stem cells in nude mice.Methods: Murine embryonic stem cells (D3 cell line) were cultured and maintained in an undifferentiated state in vitro, then transplanted into the anterior chamber of nude mice. Mophological and immunohistochemical examinations were implemented. Results: Two to three days after transplantation, yellow-white floating granules, sheets and masses were seen inside the anterior chamber and vitreous cavity, and enlarged gradually. 14 - 20 days later, the mice were executed. Morphological examination showed that there were undifferentiated cells and some round or polygonal differentiated cells in anterior chamber and vitreous cavity. The morphology of these differentiated cells were similar to that of the retina. The cells were highly positive in NSE staining. Conclusion: The tranplanted embryonic stem cells could grow in the eyes of nude mice with tendency to differentiate into neurons and r Purpose: To investigate the intraocular growth and biological characteristics of mice embryonic stem cells in nude mice. Methods: Murine embryonic stem cells (D3 cell line) were cultured and maintained in an undifferentiated state in vitro, then transplanted into the anterior chamber of nude mice Results: Two to three days after transplantation, yellow-white floating granules, sheets and masses were seen inside the anterior chamber and vitreous cavities, and enlarged gradually. 14 - 20 days later, the mice were executed Morphological examination showed that there were undifferentiated cells and some round or polygonal differentiated cells in anterior chamber and vitreous cavity. The morphology of these differentiated cells were similar to that of the retina. The cells were highly positive in NSE staining. embryonic stem cells could grow in the eyes of nude mice with deviation to differentiate into neurons and r