论文部分内容阅读
通过发散法合成了不同代数的β-环糊精为核的聚酰胺-胺树状大分子(CP),进一步与水杨醛缩合得到3种水杨醛改性的β-环糊精为核的聚酰胺-胺树状大分子(CPS),并采用1H-NMR,13C-NMR,IR对其结构进行了表征.应用荧光光谱技术研究了不同代数的CP及CPS分别与牛血清白蛋白(BSA)的相互作用,对其作用的机理进行了初步的分析与探讨.结果表明,两类树状大分子对BSA荧光的影响属于静态猝灭,且随着代数的增加,其猝灭常数Ksv增大.与类似结构的以乙二胺为核的PAMAM比较,CP及CPS的猝灭常数Ksv较大,显示出较强的结合力,表明β-CD分子作为核介入树状分子以及末端水杨醛的改性能够显著地增强分子间的相互作用.
Polyamidoamine dendrimers (CPs) with different algebraic β-cyclodextrin nuclei were synthesized by the divergent method and further three salicylaldehyde-modified β-cyclodextrins were nucleated by condensation with salicylaldehyde (CPS) was synthesized and characterized by 1H-NMR, 13C-NMR and IR.Using fluorescence spectroscopy to study the effect of different generations of CP and CPS on the structure of bovine serum albumin BSA), and its mechanism of action was initially analyzed and discussed.The results showed that two kinds of dendrimers on BSA fluorescence belongs to the static quenching, and with the increase of algebra, the quenching constant Ksv .Compared with PAMAM of similar structure with ethylenediamine as nucleus, the quenching constant Ksv of CP and CPS is larger and shows stronger binding force, indicating that β-CD molecule acts as core-intercalating dendrimer and terminal water Salicylaldehyde modification can significantly enhance the intermolecular interactions.