目的比较低密度和高密度红细胞表面磷脂酰丝氨酸(PS)、CD47、CD55和CD59的表达差异,探讨这些分子在红细胞老化及非调理性吞噬中的作用。方法用percoll配置不同的密度梯度介质(1.104、1.117、1.130g/ml),按比重由高到低依次加入玻璃离心管中。新鲜采集的红细胞用磷酸盐缓冲液(PB Objective To compare the expression of phosphatidylserine (PS), CD47, CD55 and CD59 on low density and high density erythrocytes, and to explore the role of these molecules in the aging and non-opsonization phagocytosis of erythrocytes. Methods Different density gradient media (1.104,1.117,1.130g / ml) were prepared by percoll, and then added to the glass centrifuge tube according to their specific gravity from high to low. Freshly collected red blood cells were washed with phosphate buffer (PB