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室内抗药性筛选导致褐飞虱对甲胺磷抗性的上升 ;连续筛选 9代后 ,停止抗性筛选 ,在连续 9个世代观察抗性的下降。在抗药性上升和下降的全过程中 ,对酶酶活性、多功能氧化酶活性、谷胱甘肽 S 转移酶活性和乙酰胆碱酯酶不敏感性的变化进行了研究。结果显示 ,在抗药性变化的过程中 ,酯酶活性的变化与甲胺磷对褐飞虱致死中量 (LD50 )的变化显著相关 ,表明酯酶在抗药性上升和抗药性下降过程中都起到十分重要的作用。乙酰胆碱酯酶不敏感性的变化只在抗药性上升阶段与致死中量 (LD50 )的变化显著相关 ,而多功能氧化酶活性和谷胱甘肽 S 转移酶活性的变化只在抗药性下降阶段与致死中量 (LD50 )的变化显著相关 ,表明它们在抗药性上升阶段和下降阶段的作用存在一定的差异
In-house resistance screening led to an increase of methamidophos resistance in N. lugens. After nine generations of continuous screening, resistance screening was stopped and the decline in resistance was observed for nine consecutive generations. The changes of enzyme activity, multifunctional oxidase activity, glutathione S transferase activity and the insensitivity of acetylcholinesterase were studied in the whole course of resistance rise and fall. The results showed that the change of esterase activity was significantly correlated with the lethal dose of methamidophos (LD50) during the course of drug resistance change, indicating that esterase played a very important role in the rise of drug resistance and the decline of drug resistance Important role. The change of insensitivity of acetylcholinesterase was only significantly correlated with the change of LD50 in the stage of drug resistance increase, while the changes of multifunctional oxidase activity and glutathione S transferase activity only correlated with the decrease of drug resistance There was a significant correlation between the changes of LD50, indicating that there is a certain difference between them in the rising and declining stages of drug resistance