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建立了高效液相色谱-波长切换法同时测定参苓健脾胃颗粒中的补骨脂素、花椒毒素、佛手苷内酯、欧前胡素、白术内酯Ⅲ、白术内酯Ⅰ和白术内酯Ⅱ的含量。采用Phenomenex ODS C_(18)色谱柱,以乙腈∶0.1%磷酸为流动相,梯度洗脱,波长切换检测,检测波长分别为310 nm(补骨脂素、花椒毒素、佛手苷内酯和欧前胡素)、220 nm(白术内酯Ⅰ和Ⅲ)和276 nm(白术内酯Ⅱ)。7种成分的浓度与峰面积在测定范围内线性关系良好;平均加样回收率分别为98.5%、97.6%、97.2%、99.5%、96.6%、99.6%和98.4%,RSD分别为0.93%、1.11%、1.26%、1.54%、0.65%、1.18%和1.43%。
A high performance liquid chromatography-wavelength-switching method was established for the simultaneous determination of psoralens, pricklyashin, bergapten, imperatorin, atractylenolide Ⅲ, atractylenolide I and atractylenolide Ⅱ content. Phenomenex ODS C 18 column was used with mobile phase of acetonitrile: 0.1% phosphoric acid as the mobile phase, gradient elution and wavelength switching detection. The detection wavelength was 310 nm (psoralen, pricklyashin, bergapten, Hu Su), 220 nm (atractylodes I and III) and 276 nm (atractylen II). The linearity of the seven components was linear with the peak area in the determination range. The average recoveries were 98.5%, 97.6%, 97.2%, 99.5%, 96.6%, 99.6% and 98.4%, respectively, with RSDs of 0.93% 1.11%, 1.26%, 1.54%, 0.65%, 1.18% and 1.43%.