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目的分析快速纸片法与传统发酵法在不同类型样品中检测大肠菌群的一致性。方法用快速纸片法和传统发酵法同时检测同一样品的大肠菌群,比较检验结果的一致性以及假阳性率。结果两种方法检测5类样品总符合率为88.2%(糕点95.7%、冷冻饮品82.5%、卤菜66.7%、水质100%、餐具96.4%);冷冻饮品被污染程度不同对两种检测方法的符合率有影响;纸片法的不典型性结果真阳性率只有51.2%,且在不同类型的标本中分布不同。结论使用快速纸片法试纸必须根据相对应类型的标本研制和配对使用,对于不典型结果判断需用传统方法确证。
OBJECTIVE: To analyze the consistency of the fast disk method and the traditional fermentation method for detecting coliform bacteria in different types of samples. Methods Simultaneous detection of coliform bacteria in the same sample by fast disc method and traditional fermentation method was used to compare the consistency of the test results with the false positive rate. Results The total coincidence rate of the two methods was 88.2% (95.7% for cakes, 82.5% for frozen drinks, 66.7% for braised vegetables, 100% for water quality and 96.4% for tableware). The differences of contamination degree of frozen drinks between the two methods Rate of impact; paper method atypical results of the true positive rate of only 51.2%, and in different types of specimens distributed differently. Conclusion The use of fast disc test strips must be based on the corresponding types of specimens developed and paired with the typical results for the judgment to be confirmed by traditional methods.