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目的研究三磷酸腺苷(ATP)对人食管癌细胞株Eca-109和人肝癌细胞株SMMC-7721细胞增殖的影响。方法采用MTT法测定ATP、腺苷(ADO)和三磷酸尿苷(UTP)抑制肿瘤细胞增殖的作用,W rights-G iem sa染色观察细胞形态学的改变。结果ATP(0.03~0.3 mmol.L-1)和ADO(0.1~0.3 mmol.L-1)可不同程度地抑制Eca-109和SMMC-7721的增殖,ATP对两株肿瘤细胞增殖的抑制作用均强于ADO。ATP和ADO对Eca-109细胞的最大抑制率分别为86.36%和29.88%,IC50分别为0.056和0.823 mmol.L-1;对SMMC-7721细胞的最大抑制率分别为82.06%和52.84%,IC50分别为0.218和0.517 mmol.L-1。UTP对Eca-109细胞有很弱的抑制增殖作用,最大抑制率仅为18.27%;对SMMC-7721无抗增殖作用。两株细胞经高浓度(0.3 mmol.L-1)ATP处理后,部分SMMC-7721细胞形态出现了明显的凋亡特征,而Eca-109细胞凋亡特征不明显。结论ATP具有较强的抗Eca-109细胞增殖作用,其抗增殖作用主要由ATP自身所致,代谢产物ADO也具有一定的作用;而对于SMMC-7721细胞,ATP可能较大程度地通过降解为ADO而发挥抗增殖作用。
Objective To study the effect of adenosine triphosphate (ATP) on the proliferation of human esophageal cancer cell line Eca-109 and human hepatocellular carcinoma cell line SMMC-7721. Methods MTT assay was used to determine the effects of ATP, adenosine (ADO) and uridine triphosphate (UTP) on the proliferation of tumor cells. The morphological changes of cells were observed by Wright-Giemsa staining. Results ATP (0.03-0.3 mmol.L-1) and ADO (0.1-0.3 mmol.L-1) inhibited the proliferation of Eca-109 and SMMC-7721 to some extent. The inhibitory effect of ATP on the proliferation of both tumor cells Stronger than ADO. The maximum inhibitory rates of ATP and ADO on Eca-109 cells were 86.36% and 29.88%, respectively, with IC50 of 0.056 and 0.823 mmol.L-1, respectively. The maximum inhibitory rates of ATP and ADO on Eca-109 cells were 82.06% and 52.84% Respectively 0.218 and 0.517 mmol.L-1. UTP had a weak inhibitory effect on the proliferation of Eca-109 cells, the maximum inhibitory rate was only 18.27%; no antiproliferative effect on SMMC-7721. After treated with high concentration (0.3 mmol.L-1) of ATP, the apoptosis of some SMMC-7721 cells was obvious, but the apoptosis of Eca-109 cells was not obvious. Conclusion ATP has a strong anti-proliferation effect on Eca-109 cells, and its antiproliferative effect is mainly caused by ATP itself, and the metabolite ADO also has a certain effect. For the SMMC-7721 cells, ATP may be largely degraded to ADO play an anti-proliferative effect.