论文部分内容阅读
目的观察叶下珠复方Ⅱ号对四氯化碳(CCl4)致小鼠急性肝损伤的保护作用,并探讨其作用机制。方法72只昆明小鼠(KM小鼠)随机分为正常组、模型组、叶下珠复方Ⅱ号高剂量组(6.04 g/kg)、中剂量组(3.02 g/kg)、低剂量组(0.604 g/kg)和联苯双酯组(150 mg/kg),给药7 d后,除正常组外,其余各组小鼠均按10 m L/kg腹腔注射0.12%CCl4大豆油溶液,16 h后取小鼠血清,检测丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性;取肝组织,观察肝组织病理改变,并检测超氧化物歧化酶(SOD)活性、还原性谷胱甘肽(GSH)和丙二醛(MDA)水平变化。结果与模型组比较,叶下珠复方Ⅱ号高、中剂量组可显著降低急性肝损伤小鼠血清ALT、AST活性(P<0.01或P<0.05),显著减轻肝组织病理改变,并提高肝组织SOD活性(P<0.01或P<0.05),降低肝组织MDA水平(P<0.01)。结论叶下珠复方Ⅱ号对CCl4诱导的小鼠急性肝损伤有较好的保护作用,其作用机制与抗脂质过氧化损伤有关。
Objective To observe the protective effect of Phyllanthus Compound Ⅱ on acute liver injury induced by carbon tetrachloride (CCl4) in mice and its mechanism. Methods Kunming mice (KM mice) were randomly divided into three groups: normal group, model group, Ye Xiazhu Compound Ⅱ high dose group (6.04 g / kg), medium dose group (3.02 g / kg), low dose group 0.604 g / kg) and bifendate group (150 mg / kg). After 7 days of administration, the mice in each group were injected intraperitoneally with 0.12% CCl4 soybean oil solution at a dose of 10 m L / kg, Twenty-four hours later, the serum of mice was taken and the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) was measured. The liver tissue was taken out and the pathological changes of liver were observed. The activities of superoxide dismutase ) Activity, reduced glutathione (GSH) and malondialdehyde (MDA) levels. Results Compared with the model group, the ALT and AST activities of ALDH group were significantly decreased (P <0.01 or P <0.05), and the ALT and AST levels of the ALDH group significantly reduced the pathological changes of the liver and liver (P <0.01 or P <0.05), and decreased the level of MDA in liver tissue (P <0.01). Conclusion Ye Xia Zhu Fu Fang Ⅱ CCl4-induced acute liver injury in mice have a good protective effect, its mechanism of action and anti-lipid peroxidation damage.