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目的:观察碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)对人涎腺腺样囊性癌ACC-2细胞株增殖及MEK1/2、ERK1/2及MKP-1表达的影响。方法:培养人涎腺腺样囊性癌细胞株(ACC-2),MTT比色法测定不同浓度bFGF对细胞增殖的影响;免疫沉淀法纯化蛋白并ERK试剂盒测定ERK活性;免疫印迹法测定p-MEK1/2、p-ERK1/2及MKP-1表达。结果:MTT实验显示bFGF明显增强ACC-2细胞增殖,免疫沉淀法显示bFGF上调ERK活性,免疫印迹法显示bFGF明显增强p-MEK1/2、p-ERK1/2表达及抑制MKP-1表达。结论:bFGF可促进人涎腺腺样囊性癌ACC-2细胞株增殖,其途径与上调ERK活性、激活MEK/ERK通路、抑制MKP-1表达有关。
Objective: To observe the effects of basic fibroblast growth factor (bFGF) on the proliferation and the expression of MEK1 / 2, ERK1 / 2 and MKP-1 in human salivary adenoid cystic carcinoma ACC-2 cell line. Methods: The human salivary adenoid cystic carcinoma cell line (ACC-2) was cultured. The effect of different concentrations of bFGF on the cell proliferation was determined by MTT colorimetric assay. The protein was purified by immunoprecipitation and the ERK activity was measured by enzyme-linked immunosorbent assay p-MEK1 / 2, p-ERK1 / 2 and MKP-1 expression. Results: MTT assay showed that bFGF significantly enhanced the proliferation of ACC-2 cells. Immunofluorescence showed that bFGF up-regulated the activity of ERK. Western blotting showed that bFGF significantly enhanced the expression of p-MEK1 / 2 and p-ERK1 / 2 and inhibited the expression of MKP-1. CONCLUSION: bFGF can promote the proliferation of human salivary adenoid cystic carcinoma ACC-2 cell line through up-regulating ERK activity, activating MEK / ERK pathway and inhibiting MKP-1 expression.