蜡质芽孢杆菌（Ｂａｃｉｌｕｓｃｅｒｅｕｓ）经培养后，离心收集菌体，将菌体破碎、离心、经（ＮＨ４）２ＳＯ４分级沉淀、ＳｅｐｈａｄｅｘＧ－７５凝胶过滤和ＤＥＡＥ－５２柱层析所获得的超氧化物歧化酶进行ＳＤＳ－ＰＡＧＥ电泳为一条带，亚基分子量为１７７８３Ｄ，表明制备得到了电泳纯品。将该样品经氰化钾、双氧水、氯仿－乙醇抑制实验和特征吸收光谱分析，鉴定出此酶为Ｆｅ－ＳＯＤ，比活为：１７５３．８ｕ。 After culturing Bacillus cereus, the cells were collected by centrifugation, the cells were disrupted, centrifuged, fractionated by (NH4) 2SO4, filtered through a Sephadex G-75 gel filtration column and subjected to DEAE-52 column chromatography to obtain superoxide Dismutase was subjected to SDS-PAGE electrophoresis as a band with a molecular weight of 17783D, indicating that the purified product was obtained. The sample was inhibited by potassium cyanide, hydrogen peroxide, chloroform - ethanol inhibition experiments and characteristic absorption spectroscopy, identified as Fe-SOD enzyme, the specific activity was: 1753.8u.