论文部分内容阅读
目的建立UPLC法同时测定羊耳菊Inula cappa中7种化学成分(1,3-O-二咖啡酰基奎宁酸、1,5-O-二咖啡酰基奎宁酸、3,5-O-二咖啡酰基奎宁酸、4,5-O-二咖啡酰基奎宁酸、3-O-咖啡酰基奎宁酸、4-O-咖啡酰基奎宁酸、5-O-咖啡酰基奎宁酸)的方法,并基于该方法建立羊耳菊药材指纹图谱。方法采用WATERS ACQUITY UPLC BEH C18色谱柱(100 mm×2.1mm,1.7μm),流动相为0.1%乙酸水溶液-乙腈,梯度洗脱,柱温为30℃,样品温度为4℃,体积流量为0.4 m L/min,检测波长为329 nm。结果测定了广西、贵州、云南3个省份共20批羊耳菊样品中7种成分的量,建立了羊耳菊药材指纹图谱,共确定了20个共有峰,除样品18(S18)外,样品相似度均在0.900以上。以羊耳菊中7种成分的量及与对照指纹图谱的相似度进行单因素方差分析、聚类分析和主成分分析,结果显示,仅1,3-O-二咖啡酰基奎宁酸、1,5-O-二咖啡酰基奎宁酸的量在产地间有显著差异,20批样品被聚为2类,广西壮族自治区羊耳菊质量均一性较贵州、云南省高。结论所建立的方法快速、准确,可用于羊耳菊的质量评价。
OBJECTIVE To establish a HPLC method for the simultaneous determination of seven chemical constituents in Inula cappa (1,3-O-dicavoquinoylquinic acid, 1,5-O-disofoacylquinic acid, 3,5-O- 5-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, 5-O-caffeoylquinic acid) Method, and based on the method to establish the fingerprint of medicinal Herbe. Methods A WATERS ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) was used. The mobile phase consisted of 0.1% aqueous acetic acid - acetonitrile. The column temperature was 30 ℃. The sample temperature was 4 ℃ and the volume flow rate was 0.4 m L / min, the detection wavelength is 329 nm. Results Seventeen species of Pleurotus ostreatus from three provinces in Guangxi, Guizhou and Yunnan provinces were assayed. The fingerprints of Pleurotus ostreatus were established and 20 common peaks were identified. Except for sample 18 (S18) Sample similarity is above 0.900. The results of one-way ANOVA, cluster analysis and principal component analysis showed that only 1,3-O-caffeoylquinic acid, 1 , The amount of 5-O-dis Caffeoylquinic acid was significantly different from the origin, 20 batches of samples were clustered into two groups, and the quality homogeneity of Pleurotus major in Guangxi Zhuang Autonomous Region was higher than those in Guizhou and Yunnan Provinces. Conclusion The established method is rapid and accurate and can be used to evaluate the quality of Myrica rubra.