目的研究源自白血病细胞系J6-1的突变型P2X7受体对白血病细胞Ramos的影响。方法用RT-PCR、共聚焦显微镜检测P2X7受体的表达、定位及BzATP刺激下受体的聚集;MTT法测定细胞增殖;流式细胞术检测细胞周期。结果突变型P2X7受体稳定表达于Ramos细胞细胞膜及细胞质;细胞增殖明显增强;300μmol/LBzATP增加细胞死亡率;100μmol/LBzATP诱导P2X7受体聚集。结论该突变体促进Ramos细胞增殖;激动剂诱发P2X7受体聚集,过量激动剂导致细胞死亡。 Objective To investigate the effect of mutant P2X7 receptor derived from leukemia cell line J6-1 on leukemia cells Ramos. Methods RT-PCR and confocal microscopy were used to detect the expression and localization of P2X7 receptor and BzATP-stimulated receptor aggregation. Cell proliferation was measured by MTT assay. Cell cycle was detected by flow cytometry. Results The mutant P2X7 receptor was stably expressed in the cell membrane and the cytoplasm of Ramos cells. The cell proliferation was significantly enhanced. The cell death rate was increased with 300μmol / L LBATP and the P2X7 receptor was induced by 100μmol / L LBATP. Conclusion The mutant promotes the proliferation of Ramos cells. The agonist induces the aggregation of P2X7 receptor and the excessive agonist leads to cell death.