论文部分内容阅读
目的研究究酸性黑木耳多糖(AAP)和酸性黑木耳多糖与红松球果多酚协同(AAP/PKP)对60Coγ辐射诱导氧化损伤的防护作用。方法昆明小鼠设AAP高、中、低剂量组分别灌胃200、100和50mg/(kg·d),AAP与PKP协同组高、中、低剂量组分别灌胃200+120、100+60、50+30mg/(kg·d),同时设正常对照组和模型对照组小鼠灌胃生理盐水。连续喂养30d后,60Coγ射线一次性全身均匀照射,总吸收剂量为6.0Gy,吸收剂量率为1.0Gy/min。24h后处死测定小鼠的血清、脾脏、肝脏、心脏、肾脏和脑中维生素C(VC)、维生素E(VE)、谷胱甘肽(GSH)、辅酶Q10(Co Q10)、丙二醛(MDA)的含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性。结果 AAP,AAP/PKP高剂量组均能显著提高VC、VE、GSH、Co Q10的含量,提高SOD、GSH-Px、CAT活性,降低MDA含量,与模型对照组有显著差异。结论相比AAP,AAP/PKP更能有效地清除体内自由基,显著提高60Coγ辐射诱导氧化损伤的防护能力,具有量效关系。
Objective To study the protective effects of AAP and AHA / AOP / PKP on 60Coγ-induced oxidative damage. Methods Kunming mice were given 200, 120 and 100 mg / (kg · d) AAP high, medium and low dose groups respectively. , 50 + 30mg / (kg · d), while normal control group and model control group mice were given normal saline. After continuous feeding for 30 days, the 60Coγ-ray was irradiated by the whole body at the same time with the total absorbed dose of 6.0 Gy and the absorbed dose rate of 1.0 Gy / min. The serum levels of vitamin C (VC), vitamin E (VE), glutathione (GSH), Co Q10 and malondialdehyde (MDA) in the serum, spleen, liver, heart, MDA and the content of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT). Results The AAP and AAP / PKP high-dose groups could significantly increase the content of VC, VE, GSH and Co Q10, increase the activities of SOD, GSH-Px and CAT and decrease the content of MDA, which were significantly different from the model control group. Conclusion Compared with AAP, AAP / PKP is more effective in scavenging free radicals and significantly increasing the protective ability induced by 60Coγ-irradiation, and has a dose-response relationship.