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目的:了解携带小肠结肠炎耶尔森菌HPI毒力岛基因irp-2的大肠杆菌在云南战区部队腹泻患者粪便标本中的存在情况,并对其菌株做毒力试验。方法:用PCR扩增法检测毒力岛基因irp-2,小白鼠腹腔注射检测毒力。结果:从268份腹泻病人粪便中分离的大肠杆菌菌株中,检测出携带小肠结肠炎耶尔森菌HPI毒力岛基因irp-2的18株,检出率为6.72%(18/268)。毒力试验使小白鼠发病并死亡。结论:云南战区存在携带小肠结肠炎耶尔森菌HPI毒力岛基因irp-2的大肠杆菌,该菌对部队指战员的健康具有潜在性的威胁。
OBJECTIVE: To understand the presence of Escherichia coli (E. coli) carrying Yersinia enterocolitica HPI virulence island gene irP-2 in fecal specimens of patients with diarrhea in the theater of troops in Yunnan Province and to test its virulence. Methods: The virulence island gene irp-2 was detected by PCR amplification and the mice were injected intraperitoneally to detect the virulence. Results: 18 strains of Y. enterocolitis HPI virulent island gene irp-2 were detected in Escherichia coli isolates isolated from 268 diarrhea patients. The detection rate was 6.72% (18/268). Toxicity tests make the mice develop and die. CONCLUSIONS: Escherichia coli harboring the irI-2 gene of Yersinia enterocolitica harboring the presence of Y. enterocolitica exists in Yunnan theater and poses a potential threat to the health of military officers and men.