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目的:建立同时测定慢前康合剂(赤芍、丹参、续断等)中芍药苷、丹酚酸B、川续断皂苷Ⅵ、甘草酸的含量测定方法。方法采用高效液相色谱(HPLC)波长切换法同时测定慢前康合剂中芍药苷、丹酚酸B、川续断皂苷Ⅵ、甘草酸。色谱条件为:色谱柱Amethyst-C_(18)(4.6×250 mm,5μm);流动相为:乙腈(A)-0.1%磷酸(B)梯度洗脱;流速为1 m L·min~(-1);检测波长:0~30分钟为230 nm;30~60分钟为280 nm;60~68分钟为210 nm;68~80分钟为254 nm。结果:芍药苷在0.10598~1.0598μg范围内线性关系良好,平均加样回收率为97.22%,RSD%为1.31%;丹酚酸B在0.19206~1.9206μg范围内线性关系良好,平均加样回收率为99.84%,RSD%为1.92%;川续断皂苷Ⅵ在0.28376~2.8376μg范围内线性关系良好,平均加样回收率为99.37%RSD%为1.23%;甘草酸在0.02216~0.2216μg范围内线性关系良好,平均加样回收率为96.52%RSD%为1.11%。结论:本方法可同时测定慢前康合剂中芍药苷、丹酚酸B、川续断皂苷Ⅵ、甘草酸等,具有方法简便、快速、准确等特点。
OBJECTIVE: To establish a method for the simultaneous determination of paeoniflorin, salvianolic acid B, dipsacus saponins Ⅵ and glycyrrhizic acid in Qianqiankang mixture (Radix Paeoniae Alba, Salvia miltiorrhiza, Dipsacus). Methods Paeoniflorin, salvianolic acid B, dipsacus saponin Ⅵ, and glycyrrhizic acid were determined simultaneously by high performance liquid chromatography (HPLC) wavelength switching method. The chromatographic conditions were as follows: Amethyst-C 18 (4.6 × 250 mm, 5 μm); gradient elution with acetonitrile-A 0.1% phosphoric acid (B); flow rate 1 mL · min -1 1); detection wavelength: 230 nm for 0-30 min; 280 nm for 30-60 min; 210 nm for 60-68 min; 254 nm for 68-80 min. Results: Paeoniflorin showed a good linearity in the range of 0.10598-1.0598 μg with an average recovery of 97.22% and a RSD% of 1.31%. Salvia acid B had good linearity in the range of 0.19206-1.9206 μg with an average recovery rate The average recovery was 99.37% RSD% 1.23%. The content of glycyrrhizin in the range of 0.02216 ~ 0.2216μg was linear within the range of 0.28376 ~ 2.8376μg, The relationship was good with an average recovery of 96.52% RSD% of 1.11%. Conclusion: This method can be used for the simultaneous determination of paeoniflorin, salvianolic acid B, dwarf saponin Ⅵ, glycyrrhizin, etc. In Ganqiankang Mixture. The method is simple, rapid and accurate.