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目的:检测NAC-1在膀胱癌及癌旁组织中的表达,及其与膀胱癌临床病理的相关性研究,并进一步探讨其与血管生成的关系,为阐明膀胱癌的发生发展机制提供基础。方法:采用免疫组化法检测88例膀胱癌和30例癌旁组织石蜡块中NAC-1的表达情况,用si-RNA干扰技术下调膀胱癌细胞系T-24中NAC-1的表达,然后通过q RT-PCR方法检测干扰组及对照组中促血管生成因子(VEGF、b FGF、EGFR)的表达。结果:膀胱癌组织和癌旁组织中NAC-1的阳性表达率分别为79.54%和13.33%,膀胱癌组织显著高于癌旁组织(P<0.01);NAC-1表达与膀胱癌的肿瘤级别、肌肉浸润程度及淋巴结转移显著相关(P<0.05),在高级别、肌肉浸润及淋巴结转移的膀胱癌中呈现高表达,细胞实验发现NAC-1下调能够抑制促血管生成因子(VEGF、b FGF、EGFR)的表达。结论:NAC-1作为肿瘤相关基因在膀胱癌中高表达,并与膀胱癌的临床病理密切相关,其能调节促血管生长因子,可能会在肿瘤的血管生成及转移中具有重要的作用。
OBJECTIVE: To detect the expression of NAC-1 in bladder cancer and its adjacent tissues and its relationship with the clinicopathological features of bladder cancer. To explore the relationship between NAC-1 and angiogenesis and to provide a basis for elucidating the mechanism of bladder cancer. Methods: The expression of NAC-1 in paraffin blocks from 88 cases of bladder cancer and 30 cases of para-cancerous tissue was detected by immunohistochemistry. The expression of NAC-1 in bladder cancer cell line T-24 was reduced by si-RNA interference The expression of pro-angiogenic factors (VEGF, b FGF, EGFR) in the interference group and the control group was detected by q RT-PCR. Results: The positive expression rate of NAC-1 in bladder cancer tissues and paracancerous tissues was 79.54% and 13.33%, respectively. The expression of NAC-1 in bladder cancer was significantly higher than that in adjacent tissues (P <0.01) , Muscle infiltration and lymph node metastasis (P <0.05). The expression of NAC-1 was down-regulated in high grade, myofibroblast invasion and lymph node metastasis of bladder cancer. The down-regulation of NAC-1 could inhibit the expression of VEGF, b FGF , EGFR) expression. CONCLUSIONS: NAC-1 is overexpressed as a tumor-associated gene in bladder cancer and closely related to the clinicopathological features of bladder cancer. It regulates pro-angiogenic growth factor and may play an important role in tumor angiogenesis and metastasis.